Quantitation of Aflatoxins B1, B2, G1, G2 and Ochratoxin A in Cannabis by LC Using Prominence-i and the RF-20Axs Fluorescence Detector
5 Dec 2018Aflatoxins and ochratoxins are varieties of mycotoxins produced as secondary metabolites from mold species within the genera Aspergillus. Considering the great risk to consumer health posed by the presence of mycotoxins and the possibility of mold growth in cannabis, the development of accurate testing methods for mycotoxins in cannabis are of utmost importance. This study focuses on developing a powerful detection method that allows cannabis testing labs to confidently report concentrations below regulatory limits – Aflatoxins B1, B2, G1 and G2 combined to 20 ppb and 20 ppb for Ochratoxin A alone.
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Prominence-i GPC System
World-class Small Multi Detection GPC System. A refractive index detector is generally used for GPC analysis of hydrophobic polymers. When trace-level additives that have UV absorption are contained in a polymer, both a UV detector and a photodiode array detector, which offers high-sensitivity detection, are used complementarily.By using the Prominence-i (PDA model), LabSolutions workstation, and LabSolutions GPC software (option), it is possible to simultaneously perform measurement of molecular weight distribution of a main component polymer using analysis results from the refractive index detector, and quantitative and qualitative analysis of trace-level additives using analysis results from the photodiode array detector.By incorporating a dual-temperature control function, which suppresses the effects of room temperature fluctuations, the RID-20A achieves excellent baseline stability and reproducibility. Moreover, the RID-20A significantly reduces stabilization time after being powered on. The stable baseline enables accurate analysis of molecular weight distribution. Shown below are a chromatogram and reproducibility of elution time results, over the course of five days, for an analysis of polystyrene containing additives.