ResourceLife Sciences
Purification and Immobilization of a Transaminase for the Preparation of an Enzyme Bioreactor
8 May 2014This application note describes the purification and immobilization of a histidine-tagged transaminase in E. coli using immobilized metal ion affinity chromatography (IMAC) and the ÄKTA™ pure chromatography system with UNICORN™ v6.3 software. The study, which included use of the UNICORN Design of Experiment (DoE) software, showed the proof-of-principle for the application of a Sepharose™ based enzyme bioreactor for industrial-scale synthesis of chemical precursors.
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ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Escherichia coliEnzymes