Preparation of 384-well qPCR Plates using the ACSIA PCR Edition, a PrimaDiag Automated Pipetting Platform

3 Jul 2016

This application note describes the validation, reliability and the reproducibility of a qPCR plate’s preparation by measuring the results of the subsequent qPCR analysis. Sample preparation was performed using an automated pipetting platform, the ACSIA PCR Edition. The results demonstrate the advantages over using a manual pipetting method, especially as all protocols can be easily programmed with the help of the PrimaController®II software.

ACSIA Tips

PrimaDiag

PrimaDiag provides its own brand of tips “ACSIA Tips”. This tips are high quality and autoclavable and ensure a great pipetting quality for the pipettes or its platforms. Specially designed to meet the quality requirements of ACSIA line, are also compatible with many manual pipettes The ACSIA Tips are available in two packaging: Rack:96 tips per rack 10 racks per box Refill:96 tips per plate 5 plates per refill 10 refills per box

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ACSIA PCR Edition - Automated Pipetting Platform

PrimaDiag

If you meet these needs: PCR setup in 96 and 384-wells plate Real time PCR setup in 96 and 384-wells plate Distribution Tube to plate transfer Plate to plate transfer Serial dilutions Hit picking Concentration normalization Plate compression/decompression SNP detection HLA genotyping PrimaDiag offers ACSIA XS PCR Edition. With the aim to increase your productivity, avoid contamination and improve work efficiency, PrimaDiag designed ACSIA XS PCR Edition. It is a compact and robust automated pipetting platform allowing performing all your simple protocols requiring a liquid handling. For instance, ACSIA PCR Edition can process without any human intervention the complete PCR setup in 96- or 384-well plate with our software, the PrimaController®II.ACSIA PCR Edition allows processing your complete PCR/qPCR setup in just 5 minutes. Moreover, thanks to an innovative system for the selection of the wells, it brings a very high flexibility for the fast tuning of your protocols and PCR/qPCR setup. Our automate offers you, with our pipetting heads, a higher accuracy and reproducibility. The quality of your samples at the end of the process will be greater.ACSIA XS PCR Edition is available with 8 SBS positions and integrate an interchangeable 250µl 1-channel pipetting head. Its software, the PrimaController®II, allows thanks to its realistic and interactive view and its real time help to increase your efficiency by focusing on your own tasks. Features: Automated platform designed for the PCR/qPCR setup in 96- and 384-wells plate Interchangeable pipetting head within seconds High quality pipetting with a CV lower than 2% for 1µl and lower than 0.05% for 250µl High efficiency automated platform managed by the most innovative and smartest software Add our protective enclosure for a better safety during your process Fast return on investment

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