Performing Chromatin Immunoprecipitation with Minimal Hands on Time Using the ChIPettor
22 Dec 2014This application note presents a comprehensive analysis pipeline for analyses of ChIP-seq data. A case study is then described in which stringent analysis methods for validating a novel semi-automated ChIP method is applied. An elegant and viable alternative to more costly automation systems is provided.
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ChIPettor™ System
DiagenodeThe ChIPettor™ System has everything you need for high-quality, fast, and easy chromatin immunoprecipitation for either histones or transcription factor ChIP. Contains a full set of reagents for start-to-finish ChIP starting with 100,000 to 1,000,000 cells (ideal for histone pull down), purification reagents, and a semi-automated pipettor with special resin pipet tips. The ChIPettor™ is designed to stand independently on a 96-deepwell plate while it automatically dispenses, pipets, and mixes reagents using a pre-programmed, optimized ChIP protocol. Just sit back and relax while the ChIPettor™ System delivers high yields of purified immunoprecipitated DNA with the highest degree of reproducibility.Features & Benefits: No hassle ChIP with pipette and high quality reagents Less than 60 minutes of hands-on time to complete up to 12 reactions Reproducible results each and every time Fully affordable – no capital equipment required Completely validated reagents and protocol Applications: ChIP