PCR Validation Testing Utilizing a Novel Dynamic Measurement System

When PCR validation is done correctly it provides measurements which can allow for the assessment of accuracy and uniformity of the PCR block. Not only does it provide useful information pertinent to the end product, it is a necessity for accreditation. Most methods employ static temperature collection with a single probe. Generally, single probe systems allow for an average of 90sec to achieve the target temperature. This does not allow for an acquisition of temperature as it would occur during normal cycling parameters. This poster demonstrates a temperature acquisition system which would provide temperatures in a dynamic environment using a Driftcon® system.