Orion Platform imaging for tumor-immune interactions

4 Jun 2024

Deeper understanding of the intricacies between tumor microenvironments and immune cell infiltrates is critical for creation of next generation therapies. In this scientific poster, RareCyte presents a single-step 15-plex fluorescence immunohistochemistry (IHC) protocol using the Orion platform to analyze tumor microenvironments and immune cell phenotypes across multiple tumor types. The poster highlights a study aimed to enhance biomarker analysis by utilizing formalin-fixed paraffin-embedded (FFPE) tumor microarrays. The results demonstrate that the Orion platform can efficiently detect 15 distinct biomarkers on a single slide, surpassing traditional methods limited to 6 markers. The method offers significant workflow advantages, reducing tissue and signal loss, and potentially leading to better diagnostic and therapeutic outcomes by providing deeper insights into immune surveillance and mechanisms of resistance in various cancers.