ResourceLife Sciences
Organotypic air-liquid interface 3D cultures as in vitro models
8 Apr 2026This resource outlines a culture protocol to generate human epidermal skin models using primary human keratinocytes, dermal fibroblasts, collagen-coated Millicell® cell culture inserts, and the proprietary 3dGRO™ Skin Differentiation Medium from Merck.
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Cell / Tissue CultureCell culture or tissue culture is used to study the biology of cells or tissues and to isolate cellular products in an environment which can be manipulated and well defined. Accurately control your culture environment with bioreactors or culture incubators, bind your cells to a surface or together with an extracellular matrix. Distinguish cell types with differential media or proliferate cells with certain characteristics using selective media. Enrich your media with supplements such as growth factors, sera and vitamins. Find the best cell and tissue culture products, kits and equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.3D Cell CultureCulturing cells in three dimensions allows more physiologically relevant observations to be made regarding cellular interactions and gene expression. 3D models provide a more accurate representation of cells in vivo and are more stable than 2D cultures, making them very useful for long-term studies.
