Optimized NGS Library Preparation with Acoustic Liquid Handling
8 Apr 2017Radiant Genomics and Labcyte have developed a low-volume, multiplexed next-generation sequencing (NGS) library preparation method using the Echo® 525 Liquid Handler. In addition to cost savings, they demonstrate an increase in sample-processing throughput with the Echo system due to efficient multiplexing, which would otherwise remain impractical using standard liquid handling automation. In addition, the barcoding and combinatorial pooling strategy outlined can be applied to workflows beyond NGS.
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Echo® 525 Liquid Handler
Beckman Coulter Life SciencesThe Echo 525 platform, like the other Echo liquid handler models, is designed to use sound energy to transfer reagents. Unlike the other Echo models, the Echo 525 platform transfers at a larger volume increment (25 nL). This enables the more rapid transfer of aqueous reagents required in biochemical and genomics assays setup.