ResourceLife Sciences
Optical Design of CFX96™ Real-Time PCR Detection System
1 Nov 2013In contrast to the optical design of most real-time PCR systems, the CFX96 and CFX384™ real-time PCR detection systems utilize solid-state optical technology with long-lasting light-emitting diodes for fluorescence excitation and photodiodes for detection. Therefore eliminating the need to sacrifice data collection in one of the channels to normalize to a passive reference. In this study, evidence is presented to support the methodology and show the impact of reaction volume of fluorescence chemistry on replicate samples.
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CFX96 Touch™ Deep Well Real-Time PCR Detection System with Starter Package
Bio-RadModular thermal cycler platform, includes C1000 Touch™ thermal cycler chassis, CFX96 Deep Well™ optical reaction module, CFX Manager™ software, qbasePLUS software license, cables, reagents, consumables
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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.Real-Time PCRReal-time PCR (RT-PCR) is a technique used to amplify and simultaneously quantify nucleic acids in real time. It is a key tool in gene expression analysis, pathogen detection, and diagnostics. RT-PCR allows for the detection of specific RNA or DNA sequences with high sensitivity and specificity. Browse our peer-reviewed product directory to find the best Real-Time PCR systems; compare products, check reviews, and get pricing directly from manufacturers.