On the RISE: Correlative Confocal Raman and Scanning Electron Microscopy

9 May 2017

Correlative imaging techniques are gaining more importance in many fields of applications as more characteristics of a specimen can be analyzed with one instrument. This application note describes the use of RISE (Raman Imaging and Scanning Electron) microscopy for a variety of applications including imaging of; geological samples, non-conductive organic materials, and hamster brain tissue. It also describes the ability of rise to analyze polymorphs and correlate structure with chemical phases.

RISE Microscopy - Correlative Raman Imaging and Scanning Electron Microscopy (Raman-SEM)

Oxford Instruments Raman

RISE Microscopy is a novel correlative microscopy technique that combines SEM and confocal Raman Imaging and links ultra-structural surface properties to molecular compound information. RISE Microscopy combines an SEM and a confocal Raman microscope. The confocal Raman microscope is integrated into the vacuum chamber of the electron microscope. Non-destructive Raman and SEM measurements are consecutively performed at two different positions inside this chamber using an automatic transfer stage. Calibration of the sample position ensures scanning of the same sample area in both SEM and Raman modes.The integrated RISE software carries out the required parameter adjustments and instrument alignments. The acquired results can then be correlated and the Raman and SEM images overlaid. RISE Microscopy pairs ease-of-use with exceptional analyzing benefits and is therefore suited for a wide variety of applications such as nanotechnology, materials science, and life science.Features: Quick and convenient switching between Raman and SEM measurement Automated sample transfer from one measuring position to the other Integrated software interface for user-friendly measurement control Correlation of the measurement results and image overlay No compromise in SEM and Raman imaging capabilities Applications: All fields of application that require a comprehensive and detailed sample analysis, e.g. materials science, pharmaceutical science and industry, geo science, nanotechnology, life science etc…

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