Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning

10 Jun 2012

Presented in this note is the ability to use a micro-volume vessel in conjunction with several different microplate readers for quantification of microliter volumes of biomolecules. The ability to detect common contaminants used in molecular biology techniques is also presented.

Agilent BioTek Synergy H1 Multimode Reader

Agilent Technologies

Agilent BioTek Synergy H1 is a modular multi-mode microplate reader, with monochromator-based optics and filter-based optics. The proprietary Hybrid Technology offers flexibility and sensitivity across a broad range of applications. The modular platform allows upgrading to expand functionality as your laboratory workflows change.

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Microplate Readers / DetectorsMicroplate readers are used to automate the detection and analysis of labeled or label-free components in microplates during assays or live-cell monitoring. Microplate readers are generally distinguished by their mode of detection. Types include absorbance, luminescence, fluorescence intensity, fluorescence polarization, TRF / FRET and multimode microplate readers. Microplate readers deliver a high throughput of samples by reading multiple wells simultaneously, with the 96-well format the most commonly used. As a result, microplate readers are often used in the drug discovery, bioassays, research and pharmaceutical industries for screening applications. Microplate loading can also be automated, with robotic microplate stackers to increase throughput. Find the best microplate readers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Spectral Scanning
Micro-Volume Purity Assessment of Nucleic Acids using A260/A280 Ratio and Spectral Scanning