ResourceLife Sciences
Methods for RNA sequencing
18 Mar 2026In this method guide, Illumina outlines next-generation sequencing workflows for transcriptome analysis using RNA sequencing (RNA-Seq). Find out how RNA-Seq enables sensitive, comprehensive profiling of gene expression, transcript isoforms, gene fusions, and single nucleotide variants in a single experiment. It emphasizes the use of bulk RNA-Seq for measuring average RNA expression in cell populations, making it accessible for newcomers and valuable for translational and clinical cancer research.
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Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.