Lower Cost, Higher Throughput Library Preparation with the Echo® Liquid Handler and the NuGEN Ovation Single Cell RNA-Seq System

3 Apr 2017

This proof of concept study provides validation of Ovation Single Cell RNA-Seq System miniaturization, by examining libraries, enabling broader application of transcriptome analysis with next-generation sequencing (NGS). Echo liquid handlers were utilized, due to their accuracy and precision. The combination of the Echo liquid handlers and NuGen Ovation Single Cell RNA-Seq System increased library preparation throughput whilst reducing costs.

Echo® 525 Liquid Handler

Beckman Coulter Life Sciences

The Echo 525 platform, like the other Echo liquid handler models, is designed to use sound energy to transfer reagents. Unlike the other Echo models, the Echo 525 platform transfers at a larger volume increment (25 nL). This enables the more rapid transfer of aqueous reagents required in biochemical and genomics assays setup.

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Lower Cost, Higher Throughput Library Preparation with the Echo® Liquid Handler and the NuGEN Ovation Single Cell RNA-Seq System