ResourceLife Sciences
Live Cell Biosensor Assay Used To Interrogate GPCRs
21 Nov 2013Functional, cell-based assays are essential to identify modulators of GPCRs, including those that signal via increases or decreases in cAMP. Several variants of the biosensor exist with different affinities for cAMP, some offering increased sensitivity and others offering increased dynamic range. In this application note the performance of the highest and lowest affinity variants are compared using automated methods and the highly-sensitive bottom reading capabilities of an HTS microplate reader. Sensitive detection of the 22F basal signal allows for use of this variant under a broad range of conditions, providing an assay with both superior dynamic range and sensitivity.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.gPCRLuminescenceLuminescence techniques detect and measure light emitted by substances during chemical reactions or biological processes. Widely used in assays for diagnostics, environmental monitoring, and drug screening, luminescence-based methods offer high sensitivity. Explore leading systems and products in our directory.