Large Scale Cultivation of CHO Cells in the Stirred Single-use Bioreactor BIOSTATR CultiBag STR
6 May 2011In this application note, Sartorius demonstrate the performance of the BIOSTATR CultiBag STR in the batch mode cultivation of CHO in a culture volume of 200 L. Serum free media was used for the cultivation. The particular CHO clone used for this study is the in house generated DG44 ST1-C6 expressing a human IgG. Typically, it reaches cell densities between 6-7 + 106 cells/mL when grown in bioreactors in this type of media.
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BIOSTAT® CultiBag STR Plus
Sartorius GroupInnovative Bioreactor, Sensor and Stirrer Technology The BIOSTAT® CultiBag STR Plus is a stirred single use bioreactor available from small scale to large scale cultivation up to 1000L. Its design is entirely based on the conventional gold standards coming from the stainless steel systems, allowing for easy scale-up and transfer from stainless steel solutions. The BIOSTAT® CultiBag STR Plus consists of one BIOSTAT® STR Plus Control Tower, one bag holder, a single-use CultiBag STR and superior BioPAT® MFCS/DA data logging software. Single as well as twin versions are available.It is ideal for: Cell Culture applications Industrial and academic research Process development Process optimization Pilot scale production Seed cultivation for large scale bioreactors Production scale Features: Completely single use bioreactor in traditional design Top driven stirrer with magnetic coupling Application driven fully flexible gassing system Available as single and twin version User-friendly touch screen interface for easy operation Single use optical DO & pH sensors for measurement Single use bags available with working volumes of 50L, 200L, 500L and 1000L Validation & Extractible guide available