Large Scale Cultivation of CHO Cells in the Stirred Single-Use Bioreactor BIOSTAT CultiBag STR
24 Nov 2014The BIOSTAT CultiBag STR is the only single-use bioreactor which is engineering completely on principles known from reusable bioreactors. It is completely comparable to conventional stainless steel bioreactors and fully scalable. In this study, we have demonstrated the successful batch cultivation of CHO DG44 in serum free, chemically defined media under tight feedback control of pH and DO. Cell densities of 6.3 + 106 cells/mL, which are typical for the particular clone when grown in a bioreactor, could easily be reached while maintaining good viability.
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BIOSTAT® CultiBag STR Plus
Sartorius GroupInnovative Bioreactor, Sensor and Stirrer Technology The BIOSTAT® CultiBag STR Plus is a stirred single use bioreactor available from small scale to large scale cultivation up to 1000L. Its design is entirely based on the conventional gold standards coming from the stainless steel systems, allowing for easy scale-up and transfer from stainless steel solutions. The BIOSTAT® CultiBag STR Plus consists of one BIOSTAT® STR Plus Control Tower, one bag holder, a single-use CultiBag STR and superior BioPAT® MFCS/DA data logging software. Single as well as twin versions are available.It is ideal for: Cell Culture applications Industrial and academic research Process development Process optimization Pilot scale production Seed cultivation for large scale bioreactors Production scale Features: Completely single use bioreactor in traditional design Top driven stirrer with magnetic coupling Application driven fully flexible gassing system Available as single and twin version User-friendly touch screen interface for easy operation Single use optical DO & pH sensors for measurement Single use bags available with working volumes of 50L, 200L, 500L and 1000L Validation & Extractible guide available