ResourceLife Sciences
Label-Free Cellular Assay Enables Detection of Endogenous Chemokine Receptor Activity in Human T Lymphoblastoid Cells
12 Feb 2015Corning® Epic® label-free cell assay technology utilizes a resonant waveguide grating sensor to monitor the integrated cellular responses initiated by the activation of target proteins. In this study, a label-free assay was developed to investigate the activities of an endogenously-expressed chemokine receptor CCR4 in CCRF-CEM cells.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :ChemokinesLymphocytes