L-Theanine in Green Tea
24 Nov 2014L-Theanine is a naturally occurring amino acid analog that is found in green tea. It is a psychoactive compound and there is a growing need to its pharmacological effects. The compound does not have significant UV absorption which makes adequate sensitivity in UV-based analyses problematic. Furthermore, the compound is polar and not suited to conventional reversed phase HPLC analyses. In this application note, the Diamond Hydride 2.0™ column is used to study of L-theanine in green tea. The column is designed for use in aqueous normal phase and the particle diameter is much lower than standard 4 or 5 μm phases, this increases efficiency of analyte peaks.
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Cogent Diamond Hydride™ HPLC Columns
MicroSolv Technology Corp.Cogent Diamond Hydride HPLC Columns are manufactured with TYPE-C™ silica (silica hydride) and a very small amount of carbon on the surface that adjusts the column hydrophobicity to an important level. This makes these columns have unique separation capabilities for scientists working in LCMS, metabolomics, bioanalytical, amino acids, organic acids, carbohydrates, and very polar small molecules.