ResourceLife Sciences
Intracellular Calcium Measurements Using Fluo-3 Fluorescence
Intracellular Calcium Measurements Using Fluo-3 Fluorescence
4 Dec 2015This application note reports on a highly sensitive method for measuring changes of the intracellular calcium concentration (Cai) in a renal epithelial cell line (HEK 293T) and a pituitary tumor cell line (GH4/C1) using the visible light-excitable calcium sensitive dye fluo-3 (Molecular Probes, Inc.). The basal intra-cellular fluo-3 fluorescence and the effects of substances known to elevate Cai – either via calcium in-flux or liberation from intracellular stores – were investigated. Among the substances tested were the calcium ionophore antibiotic ionomycin, ATP and extracellularly applied ethanol.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.CalciumFluorescenceThe emission of fluorescence occurs when a photon of energy is supplied to a fluorescent chemical compound by an external source, causing it to become excited. Fluorescence can be detected and measured for different purposes using microplate readers, fluorescence microscopes, fluorescence scanners, and flow cytometers.TumorsTumor research focuses on understanding abnormal cell growth that leads to cancer. Identifying biomarkers, studying tumor microenvironments, and developing targeted therapies are critical for advancing cancer treatment. Early detection and personalized treatment options are key to improving outcomes for patients. Browse our peer-reviewed product directory to explore tools for tumor research, diagnostics, and cancer therapies; compare products, read customer reviews, and get pricing directly from manufacturers.