Insights into extracellular vesicle labeling and cellular uptake using live-cell analysis
12 Aug 2024Extracellular vesicles (EVs), particularly exosomes, have received significant attention in the field of diagnostics and therapeutics due to their role in transporting biomolecules. The Incucyte® Exofluor Green EV Labeling Kit from Sartorius represents a significant advancement in the study of EVs, particularly exosomes. The kit provides a powerful platform for elucidating the biological functions of EVs by enabling researchers the ability to visualize and quantify EV uptake in real-time. Explore the kit's versatility across a variety of cell lines, EV purification methods, and functional assays.
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Incucyte® Exofluor Green EV Labeling Kit
Sartorius GroupThe Incucyte® Exofluor Green EV Labeling Kit enables non-perturbing, real-time, dynamic evaluation of cellular EV uptake and has been validated for use with the Incucyte® Live-Cell Analysis System configured with a Green | Red or Green | Orange | Near-IR optical module. Incucyte® Exofluor Green EV Labeling Dye uniformly labels the plasma membrane of small EVs (e.g., exosomes) by covalently binding to proteins and amino acids. The irreversible binding to the EVs - combined with the removal of free dye - mitigates the risk of non-specific dye attachment to other plasma membranes before or during uptake.