Improved Ion PGM® Chip Loading Reproducibility with the Eppendorf Xplorer® plus Electronic Pipette

29 May 2016

In this application note a study shows how the Eppendorf Xplorer® plus electronic pipette can significantly improve the reproducibility of the Ion Torrent® Next Generation Sequencing (NGS) workflow. This method produced reproducible results with a higher mean value and lower standard deviation.

ep Dualfilter T.I.P.S.® SealMax

Eppendorf

Don't panic - your sample is safe with ep Dualfilter T.I.P.S.® SealMax The new violet and white filter for the ep Dualfilter T.I.P.S.® SealMax filter tips combine a reliable contamination protection for your pipette from your sample liquid with virtually 100 % protection from aerosols and biomolecules.This new Dualfilter closes immediately when it comes into contact with the overpipetted sample without inhibiting valuable material. Additionally drops and splashes are retained by the white layer, the violet layer forms the highly-effective barrier against sample liquid.ep Dualfilter T.I.P.S.® SealMax Features: Reliable protection from liquids Reliable protection from aerosols and biomolecules No PCR inhibiting additives Protection of your pipette from aggressive liquids when over-aspiration occurs Available in PCR clean / Sterile (sterile and pyrogen-free) Continuous inspection of each production lot by an independent laboratory guarantees high purity and quality Lot specific purity certificates available Certificate of filter efficiency acc. to EN 1822 Applications: DNA applications (e.g. PCR) RNA applications (e.g. Gene expression analysis) Protein applications (e.g. Antibody Research) Cell Culture applications (e.g. Media) Applications with radio actives All applications which use aerosol-binding liquids

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Pipette tips - ep Dualfilter T.I.P.S.

Eppendorf

There are certain situations in the lab in which suitable safety precautions must be taken in counteract any potential risks and to prevent any unpleasant surprises. It must be possible to anticipate in advance the dangers presented by carryover effects from radioactive, highly toxic or infectious sample material as well as the contamination risk caused by foreign DNA (e.g. as a result of aerosol formation).Our ep Dualfilter T.I.P.S. have an integrated dual filter made of pure hydrophobic polyethylene without "self-sealing" additives. This filter provides protection against carryover and contamination caused by aerosols and does not swell up as a result of incorrect pipetting. Samples can then be easily recovered. In line with the special biological purity requirements of a PCR application, ep Dualfilter T.I.P.S. are "PCR clean", sterile (in accordance with USP, DAB and Ph.Eur.) and thus free of human DNA, DNase, RNase and PCR inhibitors. The new Eppendorf ep Dualfilter T.I.P.S. are the first filtertips with a two phase filter for contamination protection. The tips are manufactured to the highest possible quality from pure, non recycled materials under cleanroom conditions. The are characterized by defined flow dynamics, low wettability and high thermal stability. They are certified pyrogen free and PCR clean (free from human DNA, DNase, RNase and PCR-inhibiting substances). The two-phases effect! Two filter layers provide dual protection for pipette and sample Two filter layers provide dual protection from aerosols and biomolocules Free from PCR inhibitor additives Patent pending technology All tips are provided sterile, Eppendorf PCR clean and pyrogen-free Batch certified ep Dualfilter T.I.P.S. contains well-defined different pore sizes within the two filter layers. The white layer which faces the sample retains drops, splashes and aerosols. the blue layer above it forms a highly-effective double barrier, that reliably binds even biomolecules. Even more, ep Dualfilter T.I.P.S. are characterized by: Defined flow dynamics Low wettability High thermal stability Leak proof seal* Minimum attachment and ejection forces* *when used in combination with Eppendorf pipettes

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