hTERT-immortalized and Primary Keratinocytes Differentiate into Epidermal Structures in 3D Organotypic Culture
22 May 2017Primary and immortalized keratinocyte cultures are essential models for investigating the normal and pathological biology of the epidermis. This application notes demonstrates how an immortalized keratinocyte cell line, Ker-CT, is able to differentiate into organotypic skin equivalents in a 3D culture model.
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Corning® 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate, Bulk Packed, with Lid, Sterile, 10/Bag
Corning Life SciencesUltra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body