How to Use Corning 3D Spheroid-Qualified Primary Human Hepatocytes

7 Dec 2018

This application note describes how 3D spheroid-qualified primary human hepatocytes (PHHs) can be used to create 3D PHH spheroids for high throughput screenings or investigative studies requiring long-term culture, e.g. chronic hepatoxicity assays.

Corning® 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate, Bulk Packed, with Lid, Sterile, 10/Bag

Corning Life Sciences

Ultra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body

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