ResourceLife Sciences
Homogenous, High-Throughput Assay to Measure Guanine Nucleotide Exchange Factor Activity
29 Jun 2017Guanine nucleotide exchange factors (GEFs) positively regulate Rho GTPases by accelerating GDP dissociation, allowing formation of the active, GTP-bound complex. This poster presents a simple, generic biochemical assay for measuring GEF activity based on GDP dissociation, developed by BellBrook Labs. The increase in GDP production is determined using the Transcreener® GDP assay, which relies on selective fluorescence polarization (FP) based immunodetection of GDP.
Links
Tags
OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.ImmunoassayImmunoassays are laboratory tests that utilize antibody-antigen interactions to detect specific substances. They are widely used in diagnostics for detecting diseases, drug testing, and environmental monitoring. These assays offer rapid, sensitive, and precise detection of biomarkers, making them invaluable in clinical and research labs. Browse our peer-reviewed product directory to compare immunoassay systems, read user reviews, and get pricing directly from manufacturers.Fluorescence Based AssayFluorescence based assays are widely used in life science research and high-throughput screening to measure a broad range of cellular activities.