ResourceLife Sciences
High-Throughput Illumina® TruSeq® Nano DNA Library Construction on the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation
11 Jun 2017The manual preparation of large numbers of sequencing libraries can be labor-intensive and time-consuming, and is a bottleneck for many laboratories. This application note demonstrates how the Biomek FXP Dual-Arm Multi-96 and Span-8 Workstation (BFXP) can be used in combination with the Illumina TruSeq Nano to create as many as 96 sequence-ready libraries from as little as 100ng of DNA. This workstation provides a reliable and efficient automation solution for NGS library construction, allowing researchers to take full advantage of Illumina’s powerful Next Generation Sequencing technology.
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DNA SequencingDNA sequencing, such as sanger sequencing, is a biological technique that determines the precise order of nucleotide bases in a fragment or template of DNA. DNA sequencers and genetic analyzers are based on capillary electrophoresis, where labeled DNA fragments are electrophoretically separated by size as they migrate through a polymer. Find the best DNA sequencing products, including DNA sequencing kits, genomic libraries and genetic identity kits in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Robotic WorkstationsRobotic workstations are automated platforms enabling the user to perform high-throughput, multi-application processes, from sample preparation tasks such as PCR set-up, DNA purification and NGS library preparation, to integrated sample prep and analysis workstations. Automation makes processes consistent, fast, precise and fully walk-away. Find the best robotic workstations in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Next Generation SequencingNext-generation sequencing (NGS), also known as whole-genome sequencing, high-throughput sequencing and massive parallel sequencing, produces and analyses thousands to millions of nucleotide sequences at once. Sequencing systems operate via varying technologies depending on the manufacturer, including sequencing by synthesis, ligation, pyrosequencing, ion semiconductor and single-molecule real-time sequencing. For NGS, library preparation is paramount to successful sequencing. In this section, explore a range of library preparation kits, from targeted, amplicon-based or hybridization-based kits including epigenomic, transcriptomic and genomic workflows to fragmentation kits. Find the best next-generation sequencing products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Library GenerationLibrary generation refers to the construction of NGS libraries from RNA and DNA sources.DNADeoxyribonucleic Acid (DNA) is the main component of chromosomes and the carrier of genetic information of living organisms. Find out here about PCR, NGS, ChIP-Seq, gel imaging, and many other techniques which can be used for the analysis of DNA.Library PreparationLibrary preparation is a critical step in sequencing workflows, where DNA or RNA samples are converted into libraries for high-throughput, next generation sequencing. This step ensures accurate results and minimizes biases. Explore library preparation kits in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.NGS AutomationNGS automation streamlines next-generation sequencing workflows, increasing throughput, accuracy, and reproducibility. Automated solutions enhance productivity in genomic research, clinical diagnostics, and personalized medicine. Browse peer-reviewed tools to compare products, read reviews, and get pricing.Genetics Automation