High-speed four-camera set-up for spatial transcriptomics analysis

Spatial biology techniques are advancing rapidly as powerful tools to gain an in-depth understanding of tissues and their cellular organization. In this application note, CrestOptics presents a high-speed four-camera set-up, based on CrestOptics confocal technology, used by Dr. Bayraktar’s group in the Wellcome Sanger Institute for spatial transcriptomics analysis. Dr. Bayraktar and collaborators developed and optimized a large-scale spatial transcriptomics pipeline, consisting of automated multiplexed smFISH (single-molecule Fluorescence In Situ Hybridization), automated high-resolution imaging, single-cell and RNA spot segmentation, single-cell gene expression analysis and mapping. The laboratory uses a top-speed CrestOptics solution consisting of an X-Light V3 spinning disk confocal equipped with four cameras in order to accomplish the complex imaging steps required for this type of research.