High Sensitivity ELISA Assays for the Detection of Melamine Residuals in Milk

17 Jan 2011

This application note describes how melamine screening from milk samples can be easily performed using simple ELISA assay with high sensitivity. The total assay time is about one hour and only standard ELISA instrumentation is needed.

Thermo Scientific Multiskan GO

Thermo Fisher Scientific

The Thermo Scientific Multiskan GO UV/Vis microplate spectrophotometer offers free wavelength selection for both 96- and 384-well plates and various types of cuvettes. It features a broad wavelength range with UV area and pathlength correction, as well as fast spectral scanning; making it ideal for virtually any photometric research application, including DNA, RNA and protein analysis. The Multiskan GO can be controlled as a stand-alone with straight-forward internal software for quick and simple measurements. And even the most demanding applications can easily be set up using the intuitive Thermo Scientific SkanIt software for PC control. Both the internal and PC software are available in multiple languages including English, French, German, Spanish, Portuguese, Russian, Chinese and Japanese.Key Features: Performs endpoint, kinetic and spectral scanning assays for 96- and 384-well plates, and cuvettes Compatible with Thermo ScientificTM μDropTM Plate – a quick and easy tool for DNA and RNA assays using sample volumes down to 2 μL Fast plate measurements - full spectrum of a sample in less than 10 seconds High quality data guaranteed by extensive self-diagnostics Automation compatible Application Areas: DNA and RNA quantification and purity Protein assays Immunoassays (ELISA) Cell proliferation and cytotoxicity assays Enzyme assays Reporter gene assays

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ELISAEnzyme-linked immunosorbent assays (ELISA), also known as enzyme immunoassays (EIA), are used for the detection and quantification of proteins, peptides and antibodies in a sample. ELISA often comes in kit format, with pre-selected antibody pairs to detect specific proteins or biomarkers of diseases such as diabetes and obesity, or cardiovascular and neurological disorders. Detection is made possible with chemiluminescent, fluorescent or colorimetric substrates and detection instruments such as microplate / ELISA readers. Other kits available include: Enzyme-linked immunospot (ELISPOT) assays for the quantification of protein-producing cells and chemiluminescent immunoassays (CLIA) kits. Find the best ELISA kits and products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.
High Sensitivity ELISA Assays for the Detection of Melamine Residuals in Milk