Genotyping using Digital PCR with the LightCycler® 1536 Real-Time PCR System to Detect the IDH1 Mutation in Plasma Samples
28 May 2013Mutations in the gene encoding isocitrate dehydrogenase 1 (IDH1) have recently been identified in gliomas. IDH1 mutations have rapidly been recognized as a powerful independent prognostic factor in subjects with glioma. Subjects with tumor cells with IDH1 mutations were shown to have a longer life expectancy than subjects with a wildtype IDH1 glioma. IDH1 mutations are primarily found in gliomas, making them an excellent biomarker for this pathology. This study describes a novel genotyping assay to detect the IDH1 mutation in free circulating tumor DNA. This IDH1 genotyping assay uses digital PCR and the LightCycler® 1536 Real Time PCR System, after a first run of COLD-PCR.
Related Products
Request Quote for All Products
LightCycler® 1536 Real-Time PCR System
LightCycler® 1536 Real-Time PCR System from Roche Applied Science - High Throughput - Redefined Forge ahead into a new era in high-throughput real-time PCR with the new LightCycler® 1536 RT-PCR Instrument. Precisely engineered for miniaturization and parallelization, this latest innovation reinforces Roche Applied Science as a global expert in real-time PCR. Take advantage of over ten years of publication-proven expertise with the next generation of groundbreaking LightCycler® Instrument technology, and break free from the limitations of the past. Advantages of the LightCycler 1536 Real-Time PCR System: Increase single-run throughput fourfold Generate 1536 data points in less than 50 minutes, with consistent sensitivity and accuracy Cut your cost per data point in half Benefit from next-generation miniturization that allows lower volumes of reagents and sample Acquire raw data you can trust Maintain continuity and comparability with your legacy results, even at extremely low reaction volumes The LightCycler® 1536 RT-PCR System builds on the high-performance optical and block cycler technologies of the proven LightCycler® 480 System. New features of the LightCycler® 1536 System include a unique 1536-well plate design, streamlined software, and the next generation of premium reagents.