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Genomic DNA Isolation from Whole Blood using AcroPrep™ Advance 96-Well DNA Binding Plates on an Automated Liquid-Handling Robot

Pall Life Sciences - Laboratory, Food, Beverage
12 Apr 2012

The protocol in this note represents an example of a fully automated process for high throughput sample preparation from human blood (anticoagulated) optimized for DNA binding plates, and using commercially available buffers with a liquid handling system.

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AcroPrep™ Advance Filter Plates

Cytiva

AcroPrep™ Advance filter plates are the newest edition to Pall’s filter plate family. New sealing technology and advanced tip design make these the first choice in critical sample prep applications.

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AcroPrep™ Advance 96-Well Filter Plates for DNA Purification

Pall Life Sciences - Laboratory, Food, Beverage

Maximum Yields of High Quality DNA. Optimized for maximum binding yield of DNA from a variety of sample types New well geometry results in reduced hold-up volume and higher recovery of DNA New outlet tip geometry provides direct flow of samples into receiver plate without concerns of crosscontamination Enhanced membrane ensures high yields of contaminant-free DNA for downstream applications Manufactured in accordance with SBS guidelines, allowing entire DNA purification process to be performed on automated equipment

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Genome AnalysisGenomics, the study of genomes, includes functional genomics, evolutionary genomics and comparative genomics. There are many genomic technologies such as DNA sequencing of whole genomes, computational biology and bioinformatics. DNA and nucleic acids must be isolated and concentrated from cells for analysis with kits, automated analyzers and software. Other useful technologies for studying genomics include PCR, microarrays and electrophoresis.DNA / RNA Extraction and PurificationPurified DNA and RNA are required for numerous downstream molecular biology applications. Consequently, the importance of high-quality DNA/RNA extraction and purification equipment cannot be underestimated. Many purification kits are available and are typically optimized for nucleic acid type and source, including plasmid DNA, genomic DNA, mRNA, RNA and viral nucleic acid purification kits. Automated extraction and purification of nucleic acids can be implemented with magnetic bead separator instruments or high-throughput purification workstations. Find the best DNA/RNA extraction and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Plate ProcessingAutomated plate processing is used in microplate technologies for high-throughput, speed and efficiency. Plate processing systems include microplate handlers for moving plates, heat sealers, stackers and carousels for storage, retrieval and delivery, microplate washers and piercers. Useful features of plate processors include walk-away automation, compatibility and easy integration with microplate systems.Automated Liquid HandlingAutomated liquid handling equipment is used to mix, dilute and dispense allotted volumes of liquid into microplates and other vessels automatically. The robotic, liquid handling arms can dispense from single channel to 3456 multichannel as well as operate nanoliter dispensing, enabling high throughput of samples. Find the best automated liquid handlers in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Bioprocessing / FermentationBioprocessing is the use of biological materials to perform commercial, scientific or medical research processes. Biological materials used include cells, enzymes and organisms. Usually bioprocessing requires a batch or continuous bioreactor such as a fermentor or cell culture system. The advantages of using a reactor include high productivity, easy configuration, adjustable values and automation.DNA Binding PlatesGenomic DNA

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Genomic DNA Isolation from Whole Blood using AcroPrep™ Advance 96-Well DNA Binding Plates on an Automated Liquid-Handling Robot