ResourceLife Sciences
Fluorescence Quenching in Cell-Based Assays Can Be Dye Specific: A Case Study Using Both Fura-2 AM and Fluo-4 AM Calcium Reporter Dyes Using the FDSS6000
15 Dec 2014In this case study we screened a panel of 3500 compounds for antagonism using both Fura-2 AM and Fluo-4 AM dye loaded cells. Results reveal some compounds mediate strong inhibition, reported as no change in fluorescence upon agonist addition, against one dye-loaded cell model and not the other, suggesting dye-specific quenching.
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Cell-Based AssaysCell-based assays are used to monitor the presence, quantity and activities of a desired cellular analyte including drug molecules or biomarkers. This can reveal information on cell health (apoptosis, cytotoxicity, viability and proliferation assays), cell metabolism, cell migration and cell signaling mechanisms. Find the best cell-based assay products, kits and equipment with our peer reviewed product directory: compare products, check customer reviews and receiving pricing direct from manufacturers.Dyes