Flexible panel design for four-color immunofluorescence

This flyer demonstrates how Alexa Fluor^® 555 and Alexa Fluor^® 568 secondary antibodies can be incorporated into four-color immunofluorescence workflows. Discover how these spectrally distinct fluorophores support flexible panel design, enabling simultaneous visualization of multiple cellular targets while minimizing spectral overlap. Explore example staining configurations, emission profiles and imaging results in HEp-2 cells that illustrate how Alexa Fluor^® 555 or 568 can be combined with DAPI, Alexa Fluor^® 488 and Alexa Fluor^® 647 for effective multicolor fluorescence imaging.

Resource details:

Resource type: Flyer
Page count: 2
Read time: 3 mins