Fast PCR using PrimeSTAR® Max DNA Polymerase vs. Other High-Fidelity Polymerases

PrimeSTAR Max DNA Polymerase is a unique high fidelity enzyme that is excellent for fast PCR. Conveniently formulated as a 2X premix with reaction buffer and dNTPs, PrimeSTAR Max DNA Polymerase has the highest fidelity and fastest extension rate of any commercially available enzyme, along with extremely high sensitivity, processivity and specificity. It includes an elongation factor to provide efficient priming and extension, greatly reducing the time required for annealing and extension steps. As a result, PrimeSTAR Max DNA Polymerase can be used for exceptionally fast PCR. Since PrimeSTAR Max DNA Polymerase is configured as a premix, it allows rapid preparation of reactions and is useful for high-throughput applications. For highly accurate amplification during cloning and expression studies, structural or evolutionary analyses, PrimeSTAR Max DNA Polymerase is the high fidelity polymerase of choice. PrimeSTAR Max DNA Polymerase is also suitable for reactions that occur in the presence of excessive nucleic acid. Extraneous DNA ordinarily inhibits template amplification when using conventional polymerases because the amount of effective polymerase is limited by nonspecific binding. The superior processivity of PrimeSTAR Max DNA Polymerase prevents inhibition by excessive nucleic acid, resulting in a much higher success rate for PCR with minimal required optimization. Furthermore, the antibody-mediated hot start formulation of the polymerase prevents false initiation events from occurring during the reaction assembly.Features: Highest fidelity of any commercially available DNA polymerase Fastest extension speed means less time required for PCR cycles Convenient 2X premix including buffer and dNTPs, allowing reaction assembly in less time Antibody-mediated hot-start formulation Proven performance as reported in peer-reviewed literature Applications: Fast PCR for high throughput studies Cloning and expression studies Structure/function studies Evolutionary studies (e.g., SNP analyses)