ResourceForensics

Extraction of DNA from Hair using forensicGEM® Tissue

11 Aug 2013

Hairs found at crime scenes can be important trace evidence, and in some cases are the one and only link to a suspect. As a source of DNA, hair can prove vital in forensic human identification. Nevertheless, most methods for the extraction of DNA from hair involve a lengthy process that requires extensive manual manipulation. This application note presents a quick method for the extraction of DNA from hair using ZyGEM forensicGEM® Tissue. This method has proven to be a better alternative to other, more time-consuming protocols (for example phenol-chloroform extraction).

forensicGEM™

ZyGEM Corporation Limited

forensicGEM™ has been formulated for high-throughput DNA extraction from crime samples. The forensicGEM™ kits have been specifically designed to be compatible with high-multiplex STR profiling kits and all reagents are rigorously Quality Controlled to test for efficacy and for the absence of human DNA. ZyGEM technology is ideally suited to the large-scale batch processing of reference samples for forensic analysis. Validated by ESR New Zealand, one of the largest forensics laboratories in the world, forensicGEM™ is a rapid, high throughput processing technology for storage card and buccal swab reference samples. ZyGEM also offers a comprehensive range of optimized kits for the preparation of crime scene and CODIS samples. All forensicGEM™ kits are rigorously tested at a leading forensic laboratory for efficacy and purity. The basis of all of the kits is the forensicGEM™ proteinase, but special formulations have been developed for different substrates forensicGEM™ DNA EXTRACTION HAS MANY ADVANTAGES: Closed–tube (protects sample integrity) Hands–off (saves labor costs) One-step (maximizes yields and so increases the chance of obtaining a usable profile) Easily automated (compatible with most low cost, off-the-shelf liquid handling robots) Validated for forensic analysis

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Extraction of DNA from Hair using forensicGEM® Tissue