Extraction of Benzodiazepines From Human Urine Using ISOLUTE® SLE+ in Column Format Prior to GC-MS analysis

This application note describes the extraction of a range of benzodiazepines from human urine using ISOLUTE SLE+ supported liquid extraction columns prior to derivatization and GC-MS analysis. Benzodiazepines are psychoactive drugs used to treat various conditions including anxiety, insomnia, seizures and alcohol withdrawal. This application note details an optimized extraction protocol for the use of ISOLUTE SLE+ supported liquid 1 mL sample volume columns to extract a range of benzodiazepines from human urine. This simplified and efficient extraction method has analyte recoveries from 90% with LOQs as low as 40 ng/mL. The application has also been performed with halogenated and non-halogenated extraction solvents in the interests of providing for green chemistry.