ResourceLife Sciences
Evaluating Unfolding Reversibility using Temperature Cycle Measurements with the Prometheus and PR.TimeControl Software
17 Feb 2017The conformational stability of proteins is of central importance for a large number of commercial products, ranging from biopharmaceutics to industrial enzymes. This application note demonstrates the capabilities of the new PR.TimeControl software, which enables easy evaluation of unfolding reversibility for instruments of the Prometheus series. This can be done either by repeat cycles between two defined temperatures (“temperature cycling”), or by a repeated increase in the maximum temperature for each cycle (“incremental cycling”).
Links
Tags
AntibodiesAntibodies are used in techniques such as confocal and fluorescence microscopy, flow cytometry, ELISA, ELISPOT, immunohistochemistry, western blotting and immunopreciptation. Select specific antigen reactivity, high specific affinity, low non-specific binding, monoclonal or polyclonal, primary or secondary antibodies and associated conjugates such as an enzyme or dye for visualization.OligonucleotidesOligonucleotides are small nucleic acid polymers, usually less than 20 bases in length. Oligonucleotides can be made via enzymatic cleavage or more commonly by chemical synthesis with polymerases. Their use includes FISH, southern blots, microarrays and as primers in PCR. High fidelity synthesis kits and detection systems are available for easy production and detection, respectively.ProteomicsProteomics is the systemic bioinformatics study of proteins and amino acids, including their structure, size, function and identification. Tools used in proteomics include chromatography, blotting and gels, protein arrays, mass spectrometry and ELISA and associated analysis software. Analyzers and proteomic systems should be sensitive, high resolution, fast and may be automated for high-throughput.Software PlatformsSoftware platforms are useful for various stages of laboratory experiments from data collection to data storage and processing. For instance lab software is available for system control, data management, data analysis and qualification / validation.Protein BiologyThe analysis of protein expression, identity and function is vital for many areas of life science research and drug discovery. Some of the most commonly used techniques in protein analysis include Western blotting, electrophoresis and mass spectrometry.BiopharmaceuticalsBiopharmaceuticals are proteins and other compounds (such as nucleic acids) produced by living organisms that have uses as therapeutics or for in vivo diagnostics. The most well known example of a biopharmaceutical product, and the first to be approved for therapeutic use, was recombinant human insulin.ProteomicsProteomics refers to the analysis of proteins through a variety of different experiments in order to understand structure and functionality. Typical techniques include protein purification and mass spectrometry. Protein StructureProtein structure refers to the three-dimensional arrangement of amino acids in proteins, determining their function. Understanding protein structure is key in drug discovery, enzymology, and molecular biology. Explore protein structure analysis tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.