Efficient Method for Scaling-Up Human Mesenchymal Stem Cells using Falcon® Multi-Flasks
9 Aug 2015The use of single layer T-flasks can be time consuming, cumbersome and labor intensive for cell culture applications, requiring large numbers of cells. However, ongoing research into the use of multi-layered vessels is required to ensure environmental stability between layers which may affect the yield, homogeneity and potential functional performance of cell populations. In this application note, the success of scaling-up human mesenchymal stem cells (hMSCs) in Falcon® Multi-Flasks is accessed through cell growth and characteristics in comparison to cells grown in control T-175 flasks.
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Falcon® 875cm² Rectangular Straight Neck Cell Culture Multi-Flask, 5-layer with Vented Cap
Corning Life SciencesIncrease productivity by enabling to grow more cells faster and easier. 3- or 5-layer formats provide 525 cm2 and 875 cm2 cell growth surface area, respectively.Even distribution of media across all layers for homogeneous cell growthAbility to mix cells and reagents in the Falcon Multi-Flask saves time and reduces risk of contaminationFlexible design allows to pour or aspirate/recover cells using a pipetConsistent surface treatment for predictable scale-upLot number printed on each flask for traceabilityManufactured in compliance to cGMP standards