Easy perfusion for anchorage-dependent cell culture

29 Jan 2020

Anchorage-dependent cells, such as Vero cells, are widely used as a platform for viral vaccine production. In perfusion bioprocesses it is possible to constantly add nutrients and to remove byproducts, while retaining the cells in the bioreactor. In this application note, Eppendorf demonstrates how the BioFlo 320 vessel equipped with a spin filter for microcarrier can be used as a platform for high-density Vero cell culture, suitable for vaccine production.

Eppendorf New Brunswick™ BioFlo®/CelliGen® 510

Eppendorf

BioFlo 510 Fermentors and CelliGen 510 Bioreactors are intermediate systems ideal for pilot to small scale production processes. The modular design features a wide variety of standard options allowing this system to be flexible enough to meet most process requirements.   Eppendorf New Brunswick™ BioFlo®/CelliGen® 510 Working volumes: 10.75 L to 32.0 L Applications: Laboratory and pilot scale fermentation of aerobic and anaerobic bacteria, yeasts, and fungi; Laboratory and pilot scale cell culture of mammalian, insect, and human cell lines; Special applications such as stem cell culture, vaccine, monoclonal antibody, or biofuel/biopolymer production; Suitable for batch, fed batch, and continuous processes

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Eppendorf BioFlo® 320

Eppendorf

 The BioFlo 320 seamlessly blends beauty and utility in one all-inclusive package. A new industrial design, flexibility between interchangeable autoclavable and single-use vessels, intelligent sensors, Ethernet connectivity, and a new software package are only a few of the features that set it apart from the competition

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Easy perfusion for anchorage-dependent cell culture