Dynamics of Pneumocystis Carinii Air Shedding During Experimental Pneumocystosis

9 Jul 2018

In immunocompromised patients, the airborne-transmitted Pneumocystis jirovecii (human-derived Pneumocystis) is responsible for a severe interstitial alveolar lung disease, Pneumocystis pneumonia (PcP), which is fatal without specific treatment. This study uses the liquid medium biocollector Coriolis µ branched directly to an HEPA-filtered experimental chamber for collecting the surrounding air exhaled by individual rats experimentally infected with Pneumocystis carinii. By combining this method with a specific qPCR assay, P.carnii from the sampled air could be quantified.

Coriolis µ - Air Sampler

Bertin Technologies

The Coriolis Micro portable biological air sampler is a standard for testing air quality. Both indoors and outdoors, it’s the most efficient biological air sampler: It can take just 10 minutes to collect airborne particles using its cyclonic technology paired with a high suction rate. By generating samples in the fluid phase, Coriolis µ is compatible with rapid microbiological analysis methods.

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Dynamics of Pneumocystis Carinii Air Shedding During Experimental Pneumocystosis