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Dynamics of Pneumocystis Carinii Air Shedding During Experimental Pneumocystosis

Bertin Technologies
9 Jul 2018

In immunocompromised patients, the airborne-transmitted Pneumocystis jirovecii (human-derived Pneumocystis) is responsible for a severe interstitial alveolar lung disease, Pneumocystis pneumonia (PcP), which is fatal without specific treatment. This study uses the liquid medium biocollector Coriolis µ branched directly to an HEPA-filtered experimental chamber for collecting the surrounding air exhaled by individual rats experimentally infected with Pneumocystis carinii. By combining this method with a specific qPCR assay, P.carnii from the sampled air could be quantified.

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Coriolis+ - Air Sampler

Bertin Technologies

The Coriolis Micro portable biological air sampler is a standard for testing air quality. Both indoors and outdoors, it’s the most efficient biological air sampler: It can take just 10 minutes to collect airborne particles using its cyclonic technology paired with a high suction rate. By generating samples in the fluid phase, Coriolis µ is compatible with rapid microbiological analysis methods.

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PCR and Thermal CyclingPolymerase chain reaction (PCR) kits and thermal cyclers are used for the in vitro amplification of DNA permitting subsequent analysis and experimental procedures. Explore a range of high-quality polymerase, primers and nucleotides or simplify your workflow with a PCR mastermix. Find reverse transcription PCR (RT-PCR) and cDNA synthesis kits for RNA products and libraries. Quantitatively measure the amplification of DNA with real-time PCR (qPCR) and droplet digital PCR (ddPCR) kits and systems, and discover automated PCR setup solutions to increase throughput. Alternative DNA amplification methods also include recombinase polymerase amplification (RPA) kits. Find the best PCR kits and thermal cyclers and purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Animal ModelsThe use of non-human animals in experiments or behavorial observations. The research is conducted inside universities, medical schools, pharmaceutical companies, farms, defence establishments, and commercial facilities that provide animal-testing services to industry. It includes pure research such as genetics, developmental biology, behavioral studies, as well as applied research such as pharmaceutical testing in pre-clinical, before human, studies. Air SamplingAir sampling is described as the technique used to capture air for the subsequent purpose of analyzing, typically by thermal desorption, the presence of trace particulates, such as volatile organic compounds (VOCs).

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Dynamics of Pneumocystis Carinii Air Shedding During Experimental Pneumocystosis