Droplet Digital™ PCR: Ultra-Sensitive Validation Technology for RNA-Seq

As RNA-Seq increasingly assumes the discovery role once played by DNA expression microarrays, a highly precise and ultra-sensitive validation technology is needed to confirm its findings. Droplet Digital PCR (ddPCR™) is a digital technology that counts individual molecules with high precision and linearity over a five-log range. This application note draws comparisons between the two technologies and illustrates their inherent complementarity.