Determination of moisture in a protein sample using a portable NIR instrument

In this feasibility study the performance of a portable NIR instrument was evaluated to monitor moisture levels within a protein sample, as proof of concept to monitor lyophilized samples in a pharmaceutical process. Specifically, a multivariate PLS model was developed and evaluated for the ability to predict the levels of moisture in a casein protein. The spectra were collected in diffuse reflectance mode using a portable NIR instrument. The use of a portable instrument is also evaluated and compared with a lab based NIR instrument in order to show the utility of the portable instrument.