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Determination of Cholesterol Level in Human Serum - Enzymatic Colorimetric Method

PerkinElmer

Determination of Cholesterol Level in Human Serum - Enzymatic Colorimetric Method

5 Jan 2016

Clinical chemistry uses chemical processes to measure levels of chemical components in the blood. It is very useful for the early diagnostic of disease and for monitoring organ function. The most common specimens used in clinical chemistry are blood and urine. In this application note, the cholesterol level in human serum was determined by the enzymatic method using the LAMBDA™ 465 UV/ Vis Spectrophotometer and UV Lab™ software.

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PerkinElmer

PerkinElmer's LAMBDA 25/35/45 UV/Vis systems are easy to operate and deliver trustworthy results with the minimum of operator training. With over 12,000 units installed worldwide, our superior technology and proven build quality has proven itself under the toughest conditions. Our extensive suite of Instrument Performance Verification (IPV) tests confirms high quality performance over the lifetime of the system, and for regulated industries the Enhanced Security (ES) version of UV WinLab integrates seamless 21CFR part 11 technical compliance.LAMBDA 25Choose the LAMBDA 25 for routine UV/Vis applications including liquids analysis, pharmacopeia and regulatory tests. Range: 190 nm - 1100 nm Bandwidth: 1 nm (fixed) LAMBDA 35Choose the LAMBDA 35 for measurements on liquids, solids, pastes and powder samples and regulatory tests requiring variable bandwidths. Range: 190 nm - 1100 nm Bandwidth: 0.5 nm - 4 nm (variable) Integrating sphere option LAMBDA 45Choose the LAMBDA 45 for measurements on turbid and light-scattering liquid samples such as biological solutions and suspensions. Range: 190 nm - 1100 nm Bandwidth: 0.5 nm - 4 nm (variable) Pre-monochromator for reduced stray light

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UV-Visible SpectroscopyUltraviolet-visible (UV-Vis) spectrophotometers are used to measure the interaction of UV and visible light with a sample, including transmission, reflectance & absorbance. The two major instrument classes are single-beam or double-beam spectrophotometers. More specialized equipment includes colorimeters, spectroradiometers and refractometers. Portable and microvolume spectrophotometers are also available. For the modular spectroscopy lab, explore a range of light sources for combination with a spectrograph/spectrometer and optics. Find the best UV-Vis spectroscopy products in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.HematologyIn Haematology / Hematology, complete blood cell counts (or full blood counts) are obtained using automated blood count analyzers to enumerate blood cell types.  Hematology also encompasses haemostasis and coagulation, thrombophilia and hemophilia, plasma viscosity and ESR analysis, hemoglobinopathies, cell morphology and haematinic measurement.Clinical ChemistryBiochemistry (or clinical chemistry) involves the analysis of bodily fluids using chemical tests. Techniques used include HPLC, chromatography, spectroscopy, mass spectrometry, immunochemical, electrophoresis, turbidometric / spectrophotometric assay, MRI and ISE analysis. Tests are often carried out on plasma or serum but urine (urinalysis) and fecal specimens are also processed.EnzymesEnzymes are macromolecular biological catalysts used across life science, pharmaceutical and clinical research. Various enzymes can be purchased for research applications including ligases, high fidelity polymerases, nuclease, protease, restriction enzymes, tissue dissociation enzymes and enzyme inhibitors. Find the best enzymes in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.Clinical ChemistryClinical chemistry involves the analysis of bodily fluids, like blood and urine, to diagnose and monitor diseases. Tests in clinical chemistry provide critical information for patient care and treatment decisions. Explore clinical chemistry tools in our peer-reviewed product directory; compare products, check reviews, and get pricing directly from manufacturers.CholesterolBlood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.Human SerumHuman serum is found in blood plasma. The protein is used in a variety of applications including tissue engineering, transplantation and cell therapy.Colorimetric AssaysUrineThe analysis of urine is important in medicinal, anti-doping, forensic and life sciences applications. The ability to evaluate what someone has ingested through their urine enables fast detection and diagnosis of certain illnesses.

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