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Determination of Catechins and Phenolic Acids in Red Wine by Solid Phase Extraction and HPLC
31 Mar 2014This application note demonstrates a simple and rapid HPLC method for the analysis of nine catechins and phenolic acids in red wine. Extraction of these polar analytes was achieved on Thermo Scientific™ HyperSep™ Retain PEP material. The polyphenols in the extracts were quantified by a matrix-standard calibration, with extracts from a wine sample spiked with increasing amounts of analytes. Determination of the polyphenols was performed by HPLC, using a Thermo Scientific Accucore™ PFP HPLC column under gradient mobile phase conditions.
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UHPLC and HPLCHigh performance liquid chromatography (HPLC) and ultra high performance liquid chromatography (UHPLC), also known as UPLC, are analytical techniques used to separate, identify and quantitate components of complex mixtures including biological samples such as proteins and lipids as well as chemical mixtures of pesticides, drugs and oils. Both techniques are liquid chromatographic methods but differ by operating pressures (HPLC < 6000 psi < UHPLC ). Components of HPLC and UHPLC systems include columns, detectors, pumps, autosamplers and column heaters. Explore a range of UHPLC and HPLC columns for your specific sample needs including reverse phase, normal phase, ion exchange, HILIC, ion exclusion and size exclusion columns. For more specialized HPLC, explore FPLC, countercurrent LC and simulated moving bed systems. Find the best UHPLC and HPLC equipment in our peer reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.WineWine