Detection of Murine Regulatory T Cells on the Attune™ NxT Flow Cytometer
Detection of Murine Regulatory T Cells on the Attune™ NxT Flow Cytometer
22 Oct 2015This application note describes the use of the Attune NxT Flow Cytometer for 3-color immunophenotyping analysis of stained mouse splenocytes using the Foxp3 Transcription Factor Staining Buffer Kit, a fixation and permeabilization kit designed to work with transcription factors, like Foxp3, and other intracellular markers. Regulatory T cells come in many forms, with the most understood being those that express CD4, CD25, and Foxp3 (CD4+ CD25+ regulatory T cells). CD4+ CD25+ regulatory T cells are a specialized subpopulation of T cells that act to maintain homeostasis within the immune system by suppressing the immune response of other cells. This is an important “self-check” built into the immune system to prevent excessive reactions.
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Invitrogen™ Attune™ NxT Flow Cytometer
Thermo Fisher ScientificThe Invitrogen™ Attune™ NxT flow cytometer can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry to match the panels you are currently running. Multiple fluorescent proteins can be detected with an optional 561 nm laser. Whether you configure your system now or upgrade later, the Attune NxT flow cytometer can grow with you and your research needs. The Attune NxT flow cytometer, designed using acoustic-assisted hydrodynamic focusing, achieves sample-throughput rates of of 12.5 µL/min–1 mL/min or 12.5–1,000 µL/min—up to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/sec. Acoustic focusing allows significantly higher sample rates, which simplifies sample preparation, reduces protocol times, and minimizes cell loss. This feature is useful for no wash, no lyse immunophenotyping protocols using whole blood and for samples that are inherently low in concentration, including cerebrospinal fluid and stem cell preparations. Your next result relies on the ability of your experimental design and your instrument to deliver more—more data, more detail, and more throughput. The Attune NxT flow cytometer was designed to meet the demands of more complex experiments, offering reliable data, day after day, in a grow-with-you system. Features & Benefits: Modular design—Multiple configurations available—field upgradable. Save time—10X faster speeds with no loss in data quality. Simplified sample prep—No wash, no lyse options, non-clogging fluidics. Enables unique applications—Complex protocols on a broad range of cell types and samples.