Detection of HMT G9a with the Transcreener® EPIGEN Methyltransferase Assay

14 Jan 2015

This application note will serve as a guide for using the Transcreener® EPIGEN MethyltransferaseAssay to detect the initial velocity enzyme activity of HMT G9a with an assay window suitable for inhibitor screening and dose response measurements. It should be used as an adjunct to the Transcreener EPIGEN MT Assay.

Transcreener® AMP²/GMP² Phosphodiesterase Assays

BellBrook Labs

The Transcreener® AMP²/GMP² Phosphodiesterase Assay Kit directly detects AMP or GMP produced by PDEs, ligases, synthetases, ENPP1, and related enzymes using a mix‑and‑read FP or TR‑FRET format—no coupling steps needed. Compatible with 1–1000 µM native substrates, it delivers excellent HTS performance (Z’ ≥ 0.7, ≥100 mP shift) in a one-step, far‑red fluorescence assay.

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Detection of HMT G9a with the Transcreener® EPIGEN Methyltransferase Assay