Detection of HIF1α Expression at Physiological Levels Using NanoLuc® Luciferase and the GloMax® Discover System

  This ready-to-use multimode detection system includes: high-performance luminescence, fluorescence, UV-Visible absorbance, BRET and FRET, two-color filtered luminescence and kinetic measurement capabilities.   The GloMax® Discover System is a high-performance multimode detection instrument developed with Promega reagent chemistries to provide a simple means of detecting advanced chemistries. This instrument provides researchers superior luminescence sensitivity and dynamic range for both strong and weak bioluminescence experimental samples, as well as a seamless integration with Promega’s industry leading bioluminescent assays. GloMax® Discover also provides flexible use of filters for fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance measurements. The instrument is operated by an integrated Tablet PC, which provides quick and easy navigation through the control options. Exporting your results is made seamless with a variety of options, including exporting to your local data network. The GloMax® Discover software provides the required technical elements of a 21 CFR Part 11-compliant system to be used with the appropriate laboratory workflow. GloMax® Discover System Features: Integrated with Promega Assays - Developed and optimized with Promega cell and gene reporter assays for seamless workflow; you will be up and running faster. Easy-to-Use - Simple Tablet PC touch screen navigation with full PC capabilities and a state-of-the-art graphical user interface. Superior Performance - Broader dynamic range, better sensitivity and lower well-to-well cross talk for more usable data from your experiment. Cost-Effective - Spend less time optimizing. Connected to Your Workflow - Use as a standalone instrument or integrate into your high-throughput automated workflow; export data to your laboratory network; enable 21 CFR, Part 11 electronic signature compliance. Applications: Integration with Promega reagent chemistries for detection of fluorescence intensity, BRET, FRET, filtered luminescence and UV-visible absorbance assays. Integration into low- and medium-throughput automation workflows. Optimization with Promega cell and gene reporter assays.