Detection of Fluorescent Proteins using the Attune NxT Flow Cytometer
7 Nov 2016Fluorescent proteins are a valuable tool for researchers. A world of possibilities has been opened through their discovery and incorporation into biological research. The ability to monitor both live and dead manipulated cells allows researchers to better understand the mechanisms that regulate cellular processes. However, in order to utilize this, it is necessary to be able to analyze these processes on an individual cellular basis. Fortunately, with the increasing abilities of flow cytometers like the Attune NxT Flow Cytometer with up to 16 parameters of detection and clogresistant design, researchers can now explore what was overly difficult or simply impossible only a few years ago.
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Invitrogen™ Attune™ NxT Flow Cytometer
Thermo Fisher ScientificThe Invitrogen™ Attune™ NxT flow cytometer can be designed to accommodate the most common fluorophores and fluorescent proteins used in flow cytometry to match the panels you are currently running. Multiple fluorescent proteins can be detected with an optional 561 nm laser. Whether you configure your system now or upgrade later, the Attune NxT flow cytometer can grow with you and your research needs. The Attune NxT flow cytometer, designed using acoustic-assisted hydrodynamic focusing, achieves sample-throughput rates of of 12.5 µL/min–1 mL/min or 12.5–1,000 µL/min—up to 10 times faster than traditional hydrodynamic focusing systems and data acquisition speeds of 35,000 events/sec. Acoustic focusing allows significantly higher sample rates, which simplifies sample preparation, reduces protocol times, and minimizes cell loss. This feature is useful for no wash, no lyse immunophenotyping protocols using whole blood and for samples that are inherently low in concentration, including cerebrospinal fluid and stem cell preparations. Your next result relies on the ability of your experimental design and your instrument to deliver more—more data, more detail, and more throughput. The Attune NxT flow cytometer was designed to meet the demands of more complex experiments, offering reliable data, day after day, in a grow-with-you system. Features & Benefits: Modular design—Multiple configurations available—field upgradable. Save time—10X faster speeds with no loss in data quality. Simplified sample prep—No wash, no lyse options, non-clogging fluidics. Enables unique applications—Complex protocols on a broad range of cell types and samples.