Detection and Cross Contamination Evaluation of Roundup Ready Soy via Mechanical Shear Homogenization using the GLH-850

3 Jan 2017

Rotor stator homogenizers such as the GLH 850 are capable of disrupting a variety of sample matrices for DNA extraction with subsequent PCR analysis. This application note evaluates the GLH-850’s capability of processing a variety of soy sample matrices soy samples for the extraction of genomic DNA for the identification of Roundup Ready® soy. In conjunction, the potential for sample cross contamination from the use of a standard stainless steel generator probes was evaluated.

GLH 850 General Laboratory Homogenizer

OMNI International Inc.

The GLH 850 is an affordable, high performance instrument capable of processing virtually any laboratory sample in seconds. A powerful 850 watt motor provides maximum versatility for homogenizing, dispersing or emulsifying the largest variety of sample types and sizes in its class. The GLH 850 features an advanced, integrated, push button digital control system that ensures accuracy and repeatability. The unit’s closed loop variable speed controller (5,000 - 25,000 rpm) maintains selected speeds within +/- 100 rpm of the original set point. The digital timer can be set from seconds to minutes, and includes a 30 second countdown timer, which is ideal for remote applications in fume hoods or biosafety cabinets. A soft start feature ensures smooth processing and minimizes splashing. The GLH 850 is compatible with a wide range of interchangeable stainless steel rotor stator generator probes (sold separately), from 5 mm to 30 mm diameter, and processes samples ranging from Patented Omni TipTM Plastic Generator Probes, in both soft tissue and hard tissue versions, are available for researchers concerned about cross contamination between samples. Stainless steel generator probes are recommended for larger, tougher samples. Specially designed Hybrid Plastic/Stainless steel probes are also available.  

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Detection and Cross Contamination Evaluation of Roundup Ready Soy via Mechanical Shear Homogenization using the GLH-850