ResourceLife Sciences
Depletion of Albumin from Serum Samples Using ÄKTA™ start
8 Jan 2015Albumin comprises 55% to 65% of the total protein in serum. The abundance of this protein makes it difficult to study other serum proteins, hence removal of albumin in a single step will speed up the process and aid in easy identification and characterization of low-abundance serum proteins, such as disease-specific biomarkers. This application note demonstrates a method for quick and easy removal of albumin from human serum using the ÄKTA start chromatography system.
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Protein PurificationProtein purification is a vital step in drug discovery, therapeutics, biotech and life science research. The purification process typically involves subcellular or membrane protein extraction with cell lysis kits, separation of proteins from cell debris by filtration or spin columns, and the isolation of proteins of interest from other proteins and impurities with affinity purification (including fusion protein tags and antibody binding proteins A, G and L), immunoprecipitation or chromatographic methods, such as ion exchange, size exclusion and immobilized metal affinity chromatography. All purification methods come in multiple formats for your laboratory needs, including agarose or magnetic beads, resins, columns and filter plates. Find the best protein purification equipment in our peer-reviewed product directory: compare products, check customer reviews and receive pricing direct from manufacturers.BiomarkersBiomarkers are biological markers which can be measured and evaluated to indicate a biological state. The use of biomarkers in research and diagnosis can indicate a normal or disease state or drug response of cells / tissues. Biomarkers include genetic markers, cell surface markers such as antigens, antibodies or receptors and secreted molecules such as cytokines. An assay system is required for identification of biomarkers. :Human Serum Albumin