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Current methodologies for drugs of abuse whole blood testing

Biotage
19 Aug 2025

Extraction of drugs of abuse panels from whole blood samples can be challenging, especially when analyzing a broad panel of analytes from different classes. This white paper studies the analysis of 56 common illicit drugs in whole blood, focusing on various aspects of sample preparation, including pre-treatment of whole blood samples, extraction techniques, and automation using Biotage® Extrahera™ sample preparation workstation.

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Biotage® Extrahera™ Automated Extraction System

Biotage

Biotage® Extrahera™ is an easy-to-use automated system for SLE, SPE, PLD, and PPT in plate or column formats. With flexible volume handling, dual-flow positive pressure, and no programming required, it streamlines workflows, reduces solvent use, and frees analysts for higher-value tasks while delivering reproducible, GLP-compliant results.

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Toxicology / Drugs of Abuse TestingToxicology and Drugs of Abuse Testing is the investigation into toxic and illegal substances found at the scene of a crime or from a suspect and / or victim associated with a scene of a crime. Analyzers, immunoassays and drugs of abuse test kits and presumptive test kits are an essential aspect of toxicology investigation.Blood AnalysisThe analysis of blood is vital for many areas of life sciences and forensic investigations. Blood samples can be tested for a number of different reasons such as diagnosis, glucose levels, cholesterol and drug testing.Drugs of AbuseA drug of abuse is a drug that has been taken for non-medical purposes. Such drugs can have mind-altering effects, with some causing dependence and addiction.Forensic ToxicologyForensic toxicology is used to determine cause of death, drug use and contaminations. Toxicological evaluations are commonly used in analytical chemistry, clinical, life sciences and forensics.Drug TestingIt is essential to test the efficacy and toxicity of drugs at every stage of their development to ensure that a pharmaceutical product is fit for purpose and safe for use before reaching the stage of mass production and distribution.Sample Preparation

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Current methodologies for drugs of abuse whole blood testing