Corning® Spheroid Microplates: Spheroid Formation Protocol
14 May 2017This protocol describes a basic method for generating and culturing tumor spheroids in a 96-well spheroid microplate format. Since plating volumes and seeding densities may vary with cell type and downstream application, assay-specific optimization of conditions is recommended.
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Corning® 96-well Black/Clear Round Bottom Ultra-Low Attachment Surface Spheroid Microplate, Bulk Packed, with Lid, Sterile, 10/Bag
Corning Life SciencesUltra-low attachment surface with covalently bonded hydrogel that minimizes cell attachment, protein absorption, enzyme activation and cellular activation The surface is noncytotoxic, biologically inert and nondegradable Round bottoms with 300 µL total volume Recommended working volumes of 0.075 to 0.30 mL Sterilized by gamma radiation Novel well geometry aids formation of spheroids in center of well Optically clear round bottom with black opaque microplate body